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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/02/2021 |
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Actualizado : |
22/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LEONI, C.; DUARTE, F.; SPERONI, G.; SILVERA, M.; IRIARTE, W.; BONNECARRERE, V. |
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Afiliación : |
CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELICIA DUARTE BAREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; G. SPERONI, Departamento de Biolog??a Vegetal, Facultad de Agronom??a, Universidad de la Repu?blica, Montevideo, Uruguay.; MARIANA SILVERA ORREGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WANDA IRIARTE GRECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Etiology of pear flower bud necrosis in Uruguay. [Conference paper]. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Acta Horticulturae, February 2021, N°1303, p. 359-366. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.50 |
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ISSN : |
0567-7572 (print); 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2021.1303.50 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. |
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Contenido : |
Abstract:
Pear flower bud necrosis (PFBN) has been observed in Uruguayan orchards affecting the productivity of the trees. Severity of the problem is highly variable across years and despite the fact that it is observed in several countries, the etiology of the disease is not always clear. To elucidate the cause of PFBN, different activities were implemented over several years: surveys of pear orchards to quantify PFBN severity, morphological and histological studies of diseased buds, and isolation of potential pathogenic microorganisms from affected tissues. From the orchard surveys no clear association between climatic conditions (mean temperatures, precipitation, chilling hours) and PFBN were detected for the five years evaluated. Flower buds collected one month before flowering and fixed in formalin-ethanol-acetic acid solution showed necrosis associated with tumour development in bud primordia, and histological preparations from those tumours showed cell hypertrophy and hyperplasia. Finally, from the diseased buds we consistently isolated bacteria and no fungi. The bacteria were gram negative, oxidase positive, fluorescent on King B medium, produced indole-3-acetic acid and were positive in the tobacco hypersensitivity test. We sequenced the 16S RNA genes of the isolated bacteria to perform a taxonomic classification. We conclude that PFBN in Uruguay is caused by bacteria belonging to the Pseudomonas genus.
@ International Society for Horticultural Science. |
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Palabras claves : |
WILLIAM'S. |
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Thesagro : |
PSEUDOMONAS SP; PYRUS COMMUNIS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02505naa a2200253 a 4500
001 1061746
005 2021-02-22
008 2021 bl uuuu u00u1 u #d
022 $a0567-7572 (print); 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2021.1303.50$2DOI
100 1 $aLEONI, C.
245 $aEtiology of pear flower bud necrosis in Uruguay. [Conference paper].$h[electronic resource]
260 $c2021
500 $aArticle history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein.
520 $aAbstract: Pear flower bud necrosis (PFBN) has been observed in Uruguayan orchards affecting the productivity of the trees. Severity of the problem is highly variable across years and despite the fact that it is observed in several countries, the etiology of the disease is not always clear. To elucidate the cause of PFBN, different activities were implemented over several years: surveys of pear orchards to quantify PFBN severity, morphological and histological studies of diseased buds, and isolation of potential pathogenic microorganisms from affected tissues. From the orchard surveys no clear association between climatic conditions (mean temperatures, precipitation, chilling hours) and PFBN were detected for the five years evaluated. Flower buds collected one month before flowering and fixed in formalin-ethanol-acetic acid solution showed necrosis associated with tumour development in bud primordia, and histological preparations from those tumours showed cell hypertrophy and hyperplasia. Finally, from the diseased buds we consistently isolated bacteria and no fungi. The bacteria were gram negative, oxidase positive, fluorescent on King B medium, produced indole-3-acetic acid and were positive in the tobacco hypersensitivity test. We sequenced the 16S RNA genes of the isolated bacteria to perform a taxonomic classification. We conclude that PFBN in Uruguay is caused by bacteria belonging to the Pseudomonas genus. @ International Society for Horticultural Science.
650 $aPSEUDOMONAS SP
650 $aPYRUS COMMUNIS
653 $aWILLIAM'S
700 1 $aDUARTE, F.
700 1 $aSPERONI, G.
700 1 $aSILVERA, M.
700 1 $aIRIARTE, W.
700 1 $aBONNECARRERE, V.
773 $tActa Horticulturae, February 2021, N°1303, p. 359-366. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.50
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/04/2021 |
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Actualizado : |
27/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BRIANO-RODRÍGUEZ, C.; ROMERO, A.; LLAMBÍ, S.; BRANDA, A.; FEDERICI, M.; GIANNITTI, F.; CAFFARENA, D.; SCHILD, C.; CASAUX, M.L.; DUTRA, F. |
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Afiliación : |
CAROLINA BRIANO-RODRIGUEZ, DILAVE "Miguel C Rubino" (MGAP), Laboratorio Regional Este, Treinta y Tres, Uruguay; AGUSTÍN ROMERO, DILAVE "Miguel C Rubino" (MGAP), Laboratorio Regional Este, Treinta y Tres, Uruguay; SILVIA LLAMBÍ, Facultad de Veterinaria, Universidad de la República (UDELAR), Uruguay; ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO DUTRA QUINTELA, DILAVE "Miguel C Rubino" (MGAP), Laboratorio Regional Este, Treinta y Tres, Uruguay. |
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Título : |
Lethal and semi-lethal mutations in Holstein calves in Uruguay. [Mutações letais e semi-letais em bezerros da raça Holandesa no Uruguai.] |
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Complemento del título : |
Animal Production. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ciencia Rural, 2021, Volume 51, Issue 7, Article number e20200734. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200734 |
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ISSN : |
0103-8478; eISSN 1678-4596. |
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DOI : |
10.1590/0103-8478cr20200734 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: August 07, 2020; Accepted: November 23, 2020; Revised: February 23, 2021.
This research was funded by grant N-15156 of the uruguayan Instituto Nacional de Investigación Agropecuaria (INIA). |
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Contenido : |
ABSTRACT.
Genetic disorders in Holstein cattle are a health problem that has grown worldwide in recent years, compromising the sustainability of modern dairy production. In Uruguay, Holstein-based milk production is one of the most important sectors of the country?s economy, but high levels of inbreeding have decreased the breed?s fertility in recent decades. This study investigated the presence and diffusion of lethal and semi-lethal alleles causing embryo death, abortions, fetal malformations, and neonatal diseases in Holstein calves. Using the GeneSeek® Genomic Profiler? Bovine 50K BeadChip, we genotyped 383 calves (1?30 days-old) from 27 farms located in the main dairy region of Uruguay. Results showed a high prevalence of farms (85%) and carrier calves (21%), including one or more of the following semi-lethal or lethal alleles: Syndactylism (4.18%), brachyspina (3.39%), cholesterol deficiency haplotype (2.61%), complex vertebral malformation (2.09%), bovine leukocyte adhesion deficiency (1.04%s), and Holstein haplotypes HH1 (4.44%), HH3 (3.13%), HH4 (1.04%), and HH5 (0.26%). Most of these alleles had not been recognized previously in Uruguay. We concluded that lethal and semi-lethal mutations are widespread in the Holstein breed in Uruguay. More studies are required to determine their impact on dairy cattle fertility. © 2021, Universidade Federal de Santa Maria. All rights reserved.
RESUMO.
Os distúrbios genéticos nos bovinos da raça Holandesa são um problema de saúde que cresceu nos últimos anos a nível mundial, comprometendo a sustentabilidade da produção leiteira moderna. No Uruguai, a produção leiteira com base na raça Holstein é um dos setores mais importantes da economia do país, mas altos níveis de endogamia diminuíram a fertilidade da raça nas últimas décadas. O objetivo deste estudo foi investigar a presença e difusão de alelos letais e semi-letais causando morte de embriões, abortos, malformações fetais e doenças neonatais em bezerros da raça Holandesa. Usando o BeadChip Bovino 50K GeneSeek® Genomic Profiler?, genotipamos 383 bezerros (menos de um mês) de 27 fazendas localizadas na principal região leiteira do Uruguai. Os resultados mostraram uma alta prevalência de fazendas (85%) e bezerros portadores (21%), incluindo um ou mais dos seguintes alelos letais ou semi-letais: sindactilismo (4,18%), braquipespina (3,39%), haplótipo de deficiência de colesterol (2,61%), malformação vertebral complexa (2,09%), deficiência de adesão de leucócitos bovinos (1,04% s) e haplótipos de Holstein HH1 (4,44%), HH3 (3,13%), HH4 (1,04%) e HH5 (0,26%). A maioria desses alelos não havia sido reconhecida anteriormente no país. Concluímos que as mutações letais e semi-letais são comuns na raça Holstein no Uruguai. Mais estudos são necessários para determinar seu impacto na fertilidade do gado leiteiro. MenosABSTRACT.
Genetic disorders in Holstein cattle are a health problem that has grown worldwide in recent years, compromising the sustainability of modern dairy production. In Uruguay, Holstein-based milk production is one of the most important sectors of the country?s economy, but high levels of inbreeding have decreased the breed?s fertility in recent decades. This study investigated the presence and diffusion of lethal and semi-lethal alleles causing embryo death, abortions, fetal malformations, and neonatal diseases in Holstein calves. Using the GeneSeek® Genomic Profiler? Bovine 50K BeadChip, we genotyped 383 calves (1?30 days-old) from 27 farms located in the main dairy region of Uruguay. Results showed a high prevalence of farms (85%) and carrier calves (21%), including one or more of the following semi-lethal or lethal alleles: Syndactylism (4.18%), brachyspina (3.39%), cholesterol deficiency haplotype (2.61%), complex vertebral malformation (2.09%), bovine leukocyte adhesion deficiency (1.04%s), and Holstein haplotypes HH1 (4.44%), HH3 (3.13%), HH4 (1.04%), and HH5 (0.26%). Most of these alleles had not been recognized previously in Uruguay. We concluded that lethal and semi-lethal mutations are widespread in the Holstein breed in Uruguay. More studies are required to determine their impact on dairy cattle fertility. © 2021, Universidade Federal de Santa Maria. All rights reserved.
RESUMO.
Os distúrbios genéticos nos bovinos da raça Holandesa são um problema de saúde ... Presentar Todo |
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Palabras claves : |
BLAD; Bovine hereditary diseases; Brachyspina; Braquipespina; Cholesterol deficiency; Complex vertebral malf; Deficiência de colesterol; Doenças hereditárias; Haplótipos holandeses; Holstein haplotypes; Syndactyly. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
https://www.scielo.br/pdf/cr/v51n7/0103-8478-cr-51-07-e20200734.pdf
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Marc : |
LEADER 04383naa a2200397 a 4500
001 1061998
005 2021-04-27
008 2021 bl uuuu u00u1 u #d
022 $a0103-8478; eISSN 1678-4596.
024 7 $a10.1590/0103-8478cr20200734$2DOI
100 1 $aBRIANO-RODRÍGUEZ, C.
245 $aLethal and semi-lethal mutations in Holstein calves in Uruguay. [Mutações letais e semi-letais em bezerros da raça Holandesa no Uruguai.]$h[electronic resource]
260 $c2021
500 $aArticle history: Received: August 07, 2020; Accepted: November 23, 2020; Revised: February 23, 2021. This research was funded by grant N-15156 of the uruguayan Instituto Nacional de Investigación Agropecuaria (INIA).
520 $aABSTRACT. Genetic disorders in Holstein cattle are a health problem that has grown worldwide in recent years, compromising the sustainability of modern dairy production. In Uruguay, Holstein-based milk production is one of the most important sectors of the country?s economy, but high levels of inbreeding have decreased the breed?s fertility in recent decades. This study investigated the presence and diffusion of lethal and semi-lethal alleles causing embryo death, abortions, fetal malformations, and neonatal diseases in Holstein calves. Using the GeneSeek® Genomic Profiler? Bovine 50K BeadChip, we genotyped 383 calves (1?30 days-old) from 27 farms located in the main dairy region of Uruguay. Results showed a high prevalence of farms (85%) and carrier calves (21%), including one or more of the following semi-lethal or lethal alleles: Syndactylism (4.18%), brachyspina (3.39%), cholesterol deficiency haplotype (2.61%), complex vertebral malformation (2.09%), bovine leukocyte adhesion deficiency (1.04%s), and Holstein haplotypes HH1 (4.44%), HH3 (3.13%), HH4 (1.04%), and HH5 (0.26%). Most of these alleles had not been recognized previously in Uruguay. We concluded that lethal and semi-lethal mutations are widespread in the Holstein breed in Uruguay. More studies are required to determine their impact on dairy cattle fertility. © 2021, Universidade Federal de Santa Maria. All rights reserved. RESUMO. Os distúrbios genéticos nos bovinos da raça Holandesa são um problema de saúde que cresceu nos últimos anos a nível mundial, comprometendo a sustentabilidade da produção leiteira moderna. No Uruguai, a produção leiteira com base na raça Holstein é um dos setores mais importantes da economia do país, mas altos níveis de endogamia diminuíram a fertilidade da raça nas últimas décadas. O objetivo deste estudo foi investigar a presença e difusão de alelos letais e semi-letais causando morte de embriões, abortos, malformações fetais e doenças neonatais em bezerros da raça Holandesa. Usando o BeadChip Bovino 50K GeneSeek® Genomic Profiler?, genotipamos 383 bezerros (menos de um mês) de 27 fazendas localizadas na principal região leiteira do Uruguai. Os resultados mostraram uma alta prevalência de fazendas (85%) e bezerros portadores (21%), incluindo um ou mais dos seguintes alelos letais ou semi-letais: sindactilismo (4,18%), braquipespina (3,39%), haplótipo de deficiência de colesterol (2,61%), malformação vertebral complexa (2,09%), deficiência de adesão de leucócitos bovinos (1,04% s) e haplótipos de Holstein HH1 (4,44%), HH3 (3,13%), HH4 (1,04%) e HH5 (0,26%). A maioria desses alelos não havia sido reconhecida anteriormente no país. Concluímos que as mutações letais e semi-letais são comuns na raça Holstein no Uruguai. Mais estudos são necessários para determinar seu impacto na fertilidade do gado leiteiro.
653 $aBLAD
653 $aBovine hereditary diseases
653 $aBrachyspina
653 $aBraquipespina
653 $aCholesterol deficiency
653 $aComplex vertebral malf
653 $aDeficiência de colesterol
653 $aDoenças hereditárias
653 $aHaplótipos holandeses
653 $aHolstein haplotypes
653 $aSyndactyly
700 1 $aROMERO, A.
700 1 $aLLAMBÍ, S.
700 1 $aBRANDA, A.
700 1 $aFEDERICI, M.
700 1 $aGIANNITTI, F.
700 1 $aCAFFARENA, D.
700 1 $aSCHILD, C.
700 1 $aCASAUX, M.L.
700 1 $aDUTRA, F.
773 $tCiencia Rural, 2021, Volume 51, Issue 7, Article number e20200734. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200734
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